|
| Status |
Public on Dec 31, 2010 |
| Title |
Ffujikuroi_vel1_120h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Fusarium fujikuroi IMI58289 delta vel1
|
| Organism |
Fusarium fujikuroi |
| Characteristics |
genotype variation: Ffvel1 mutant
growth time: 72 hrs
|
| Growth protocol |
Fusarium fujikuroi wild type IMI58289 and Ffvel1 mutant strains were grown in liquid ICI (Imperial Chemical Industries Ltd., UK) media containing 6 mM glutamine (10 % ICI medium).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNAgents total RNA isolation kit (Promega GmbH, Mannheim, Germany).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI, USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA, following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI, USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Ffvel1 mutant
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
May 02, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Daren W. Brown |
| E-mail(s) |
Daren.Brown@usda.gov
|
| Phone |
309-681-6230
|
| Organization name |
USDA-ARS
|
| Department |
Bacterial Foodborn Pathogens and Mycology Research
|
| Street address |
1815 N. University St.
|
| City |
Peoria |
| State/province |
IL |
| ZIP/Postal code |
61604 |
| Country |
USA |
| |
|
| Platform ID |
GPL8781 |
| Series (1) |
| GSE21623 |
Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time. |
|
