|
| Status |
Public on Jun 01, 2010 |
| Title |
Astrocytes_R1 vs. Oligodendrocytes_R1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Astrocytes_R1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: Astrocytes
|
| Treatment protocol |
No treatment but different cell types were analysed.
|
| Growth protocol |
Preparation of mouse oligodendrocyte primary cultures was performed as described previously (Trajkovic et al., 2006). After 10 to 14 days, oligodendrocytes were shaken off the mixed glial cultures. Afterwards the cultures without oligodendrocytes, but containing astrocytes and microglia, were maintained in DMEM with 10% FCS. 7-10 days after shaking of oligodendrocytes another shaking of the culture released the microglia from the astrocyte layer. Microglia were harvested at this point. Primary astrocytes were harvested by trypsination after oligodendrocytes and microglia were shaken off.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Low RNA input Fluor linear Amp Kit 5184-3523(Agilent Technologies)following manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
Oligodendrocytes_R1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: Oligodendrocytes
|
| Treatment protocol |
No treatment but different cell types were analysed.
|
| Growth protocol |
Preparation of mouse oligodendrocyte primary cultures was performed as described previously (Trajkovic et al., 2006). After 10 to 14 days, oligodendrocytes were shaken off the mixed glial cultures. Afterwards the cultures without oligodendrocytes, but containing astrocytes and microglia, were maintained in DMEM with 10% FCS. 7-10 days after shaking of oligodendrocytes another shaking of the culture released the microglia from the astrocyte layer. Microglia were harvested at this point. Primary astrocytes were harvested by trypsination after oligodendrocytes and microglia were shaken off.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Low RNA input Fluor linear Amp Kit 5184-3523(Agilent Technologies)following manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2505B scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
Astrocytes1 vs. Oligodendrocytes_R1
|
| Data processing |
Agilent Feature Extraction Software (version A.8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
May 12, 2010 |
| Last update date |
Jun 01, 2010 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Pathology
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE21797 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster (mRNA profiling) |
| GSE21801 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster |
|
