|
| Status |
Public on Jun 01, 2010 |
| Title |
Immature oligodendrocytes d1_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Immature oligodendrocytes d1_Rep2
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: CNP-Cre
cell type: oligodendrocytes
time point: D1
differentiation status: Immature
|
| Treatment protocol |
No treatment but different time points were analyzed.
|
| Growth protocol |
Preparation of mouse oligodendrocyte primary cultures were performed as described previously (Simons et al., 2000). After shaking, cells were plated in Dulbecco's modified Eagle's medium (DMEM) with B27 supplement, 1% horse serum, L-thyroxine, tri-iodo-thyronine, glutamine and pyruvate on poly-L-lysinecoated dishes or glass coverslips.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Refer to Agilent miRNA labeling protocol
|
| |
|
| Hybridization protocol |
Refer to Agilent miRNA hybridisation protocol
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B). Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
Immature oligodendrocytes d1_Rep2
|
| Data processing |
Data were processed using alogorithms specified by the Agilent Feature Extraction software (version A.8.5.1.1). Subsequent statistical analysis (detection of candidates using linear models) of data were carried out using R.
|
| |
|
| Submission date |
May 12, 2010 |
| Last update date |
Jun 01, 2010 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Pathology
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10384 |
| Series (2) |
| GSE21798 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster (miRNA Agilent Part) |
| GSE21801 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster |
|
