|
| Status |
Public on Sep 01, 2011 |
| Title |
CRC-87 |
| Sample type |
RNA |
| |
|
| Source name |
colorectal cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: microdissected colorectal cancer cells
sample type: tumor
sample identifier: Kita-392T
gender: M
age: 78
tumor-nodes-metastasis (tnm) classification - t: 4
tumor-nodes-metastasis (tnm) classification - n: 0
tumor-nodes-metastasis (tnm) classification - m: 0
fstage: 2
tnm (stage): 2B
dukes: B
his type: mod
depth: pSE
|
| Treatment protocol |
Laser micro-dissection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with QIAGEN RNeasy minElute Spin Column (QIAGEN, Inc., Valencia, CA) according to the manufacturer's protocols.
|
| Label |
Cy3
|
| Label protocol |
Cyanine (Cy)-labeled cRNA was prepared using T7 linear amplification as described in the Agilent Low RNA Input Fluorescent Linear Amplification Kit Manual (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridization was carried out using the conditions specified in the Agilent Human Whole Genome Expression system.
|
| Scan protocol |
Fluorescence intensities were determined with an Agilent DNA Microarray Scanner.
|
| Description |
Kita-392T
|
| Data processing |
Expression levels were quantified by G2567AA Feature Extraction Software Version A.7.5.1 (Agilent Technologies), which used the LOWESS (locally weighted linear regression curve fit) normalization method. Further analyses were performed using GeneSpring version 7.3.
|
| |
|
| Submission date |
May 13, 2010 |
| Last update date |
Jan 23, 2012 |
| Contact name |
Koshi Mimori |
| E-mail(s) |
kmimori@beppu.kyushu-u.ac.jp
|
| Phone |
81-977-27-1650
|
| Fax |
81-977-27-1651
|
| Organization name |
Kyushu university
|
| Department |
Department of Surgery
|
| Lab |
Kyushu Univeristy Beppu Hospital
|
| Street address |
4546 Tsurumihara
|
| City |
Beppu |
| State/province |
Oita |
| ZIP/Postal code |
874-0838 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE21815 |
Gene expression profiles in 132 laser microdissected colorectal cancer tissues |
| GSE35279 |
Gene expression profiles in 74 laser microdissected colorectal cancer tissues |
|
