|
Status |
Public on Oct 06, 2010 |
Title |
canine retina, healthy, biol. rep5 |
Sample type |
RNA |
|
|
Source name |
adult canine retina
|
Organism |
Canis lupus familiaris |
Characteristics |
breed: Beagle age: 4 years tissue: retina disease state: healthy
|
Treatment protocol |
Canine eyes were enucleated and immediately immersed in RNAlater (Ambion). Eye were stored at -80C until retinal isolation and RNA extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
Retina was collected and RNA was extracted using Rneasy columns (Qiagen) including on-column DNA digests
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
|
|
Hybridization protocol |
cRNA was hybridized overnight to Affymetrix Canine 2.0 arrays in a Model 640 hybridization oven. Chips were washed using a Model 450 GeneChip fluidics station.
|
Scan protocol |
Data were obtained using a Model 3000 GeneArray scanner.
|
Description |
n/a
|
Data processing |
RMAexpress
|
|
|
Submission date |
May 17, 2010 |
Last update date |
Oct 06, 2010 |
Contact name |
Markus H Kuehn |
Organization name |
University of Iowa
|
Department |
Ophthalmology and Visual Sciences
|
Street address |
375 Newton Road
|
City |
Iowa City |
State/province |
IA |
ZIP/Postal code |
52242 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (1) |
GSE21879 |
Neuroinflammation in Advanced Canine Glaucoma |
|