Phalanx Labeling Protocol: RNA was converted to double-stranded cDNA and amplified using in vitro transcription that included amino-allyl UTP, and the amplified RNA product was subsequently conjugated with Cy5™ NHS ester (GE Healthcare Lifesciences, Piscataway, NJ)
Hybridization protocol
Phalanx Hybridization Protocol: hybridization at 42 C overnight using the HybBag mixing system with 1X OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/ml sheared salmon sperm DNA (Promega), at a concentration of 0.025 mg/ml labeled target
Scan protocol
Raw intensity signals for each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software
Description
Vector Clone H, rep3
Data processing
Median intensity, background subtracted, then quantile normalized.
