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Status |
Public on Mar 17, 2023 |
Title |
mC-2i-rep1 |
Sample type |
SRA |
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Source name |
R1
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Organism |
Mus musculus |
Characteristics |
cell line: R1 growth protocol: N2B27+Lif+PD0325901+CHIR99021 treatment: control cell type: ES cells
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Treatment protocol |
We used ML-792, a highly selective inhibitor of the SUMO E1 enzyme, to decrease the global level of SUMOylation in mouse ESCs, with two rounds of 48 h treatments (2.5mM) between D1 and D3, D8 and D10
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Growth protocol |
Serum+Lif (D1, D3, D8, D10), N2B27+Lif+PD0325901+CHIR99021 (2i)
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Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was purified using the Quick-DNA Midiprep plus kit from Zymo Research (#D4075) The library construction was performed using the NEBNext Enzymatic Methyl-seq kit from New England Biolabs (#E7120S).
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Library strategy |
Bisulfite-Seq |
Library source |
genomic |
Library selection |
RANDOM |
Instrument model |
Illumina HiSeq 4000 |
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Data processing |
Bisulfite libraries were processed with the Bismark pipeline1 using bowtie2 aligner2 with the default parameters. Genome_build: mm10 Supplementary_files_format_and_content: tabular file (chrom, start, strand, reads methyl, reads unmethyl)
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Submission date |
Jul 21, 2021 |
Last update date |
Mar 17, 2023 |
Contact name |
Yann Loe-Mie |
E-mail(s) |
Yann.loe-mie@pasteur.fr
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Organization name |
Institut Pasteur
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Department |
Computational Biology
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Lab |
Bioinformatics and Biostatistics HUB
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Street address |
25 rue du Dr Roux
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City |
Paris |
ZIP/Postal code |
75015 |
Country |
France |
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Platform ID |
GPL21103 |
Series (2) |
GSE180489 |
HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure |
GSE180597 |
HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq] |
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Relations |
BioSample |
SAMN20343717 |
SRA |
SRX11516580 |
Supplementary file |
Size |
Download |
File type/resource |
GSM5465748_mC-2i-1_CG.bed.gz |
91.9 Mb |
(ftp)(http) |
BED |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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