|
| Status |
Public on Dec 01, 2012 |
| Title |
13813_21 |
| Sample type |
RNA |
| |
|
| Source name |
mutant infected root
|
| Organism |
Medicago truncatula |
| Characteristics |
line: a17
age: 3 day
bacterial strain: GRS138
bacterial genotype: GALA7 mutant
|
| Treatment protocol |
Name:GRS138 - nothing - pathogen infection,ralstonia solanacearum strain grs138:time 13hour . - 3 plates of 12 roots/plate were inoculated with 300ul inoculum/root. - Inoculum 10^7cfu/ml
|
| Growth protocol |
roots - - germination on water agar (4 days 4°C; 1 day 14°C) - root growth on Fahraeus medium with N-source; 3 days pre-culture in vitro before inoculation in vitro - 25°C; 75% RH;
|
| Extracted molecule |
total RNA |
| Extraction protocol |
13813_21:53ug. Standard Affymetrix Protocol
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug. Standard Affymetrix Protocol
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
We have identified two essential virulence determinants (GALA7, a type III secretion effector and HpaP, a chaperone-like protein) of Ralstonia solanacearum for the infection and colonisation of the host plant Medicago truncatula1,2. The scope of this project is to identify the GALA7 and HpaP-specific transcriptome alteration. For this purpose wild type and mutant infected root material (13h and 72h postinfection) will be analysed on M. truncatula affymetrix chips
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
May 25, 2010 |
| Last update date |
Dec 01, 2012 |
| Contact name |
stephanie huguet |
| E-mail(s) |
huguet@evry.inra.fr
|
| Organization name |
inra
|
| Street address |
2 rue gaston cremieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE21985 |
Ralstonia solanacearum effector-specific effect on Medicago truncatula trancriptome |
|
