|
| Status |
Public on May 26, 2010 |
| Title |
BW25113 |
| Sample type |
RNA |
| |
|
| Source name |
Microaerobic growth, M9 media, exponential phase
|
| Organism |
Escherichia coli |
| Characteristics |
strain: BW25113
genotype/variation: Wild type
|
| Growth protocol |
E. coli BW25113 wild type and ldhA-pflB- double mutant growing microaerobically in M9 media containing 10 g/L Glucose were harvested in the mid-exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen's RNeasy kit following manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridized onto GeneChip Antisense Genome Array using Manufacturer's protocol
|
| Scan protocol |
Scanned using GeneArray Scanner (Affymetrix) using manufacturer's protocol
|
| Description |
Gene expression data
|
| Data processing |
Affymetrix MAS5 Normalization routine
|
| |
|
| Submission date |
May 25, 2010 |
| Last update date |
May 25, 2010 |
| Contact name |
Amar Singh |
| E-mail(s) |
singha@colorado.edu
|
| Organization name |
University of Colorado
|
| Department |
Chemical & Biological Engineering
|
| Street address |
1111 Engineering Drive, UCB 424
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309 |
| Country |
USA |
| |
|
| Platform ID |
GPL199 |
| Series (1) |
| GSE21995 |
Gene expression profile of an ldhA-pflB- double knockout strain of E. coli BW25113 relative to wild-type |
|
