|
| Status |
Public on Jan 06, 2023 |
| Title |
TL9-65 kcal |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
TL9-Radiation-induced thymic lymphoma in 65 kcal group
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1
Sex: Male
calorie: 65 kcal/week
tissue: Thymic lymphoma
|
| Treatment protocol |
B6C3F1 male mice were exposed to 3.8 Gy X-rays at 1 week of age, and then separated into different calorie-intake groups (95 kcal/mouse/week or 65 kcal/mouse/week) from 7 weeks of age.
|
| Growth protocol |
Mice were separated into different calorie-intake groups from 7 weeks of age. Mice were observed daily until moribund, when thymic enlargement and dyspnea symptoms were recognized, after which they were sacrificed by exsanguination under terminal isofluorane anesthesia and autopsied.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from 48 mice ears and lymphomas using Maxwell® 16 Instrument (Promega). After extraction, DNA was treated with RNase A (Sigma).
|
| Label |
Cy5
|
| Label protocol |
Alu I- and Rsa I-restricted DNA (400 ng) derived from ear and lymphoma were labeled by 60 µM Cyanine3-UTP and Cyanine5-UTP, respectively. Exo-Klenow fragment enzyme (Agilent Genomic DNA Enzymatic Labeling kit) was used to label these DNA.
|
| |
|
| Channel 2 |
| Source name |
Ear obtained from the same individual
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1
Sex: Male
calorie: 65 kcal/week
tissue: Ear
|
| Treatment protocol |
B6C3F1 male mice were exposed to 3.8 Gy X-rays at 1 week of age, and then separated into different calorie-intake groups (95 kcal/mouse/week or 65 kcal/mouse/week) from 7 weeks of age.
|
| Growth protocol |
Mice were separated into different calorie-intake groups from 7 weeks of age. Mice were observed daily until moribund, when thymic enlargement and dyspnea symptoms were recognized, after which they were sacrificed by exsanguination under terminal isofluorane anesthesia and autopsied.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from 48 mice ears and lymphomas using Maxwell® 16 Instrument (Promega). After extraction, DNA was treated with RNase A (Sigma).
|
| Label |
Cy3
|
| Label protocol |
Alu I- and Rsa I-restricted DNA (400 ng) derived from ear and lymphoma were labeled by 60 µM Cyanine3-UTP and Cyanine5-UTP, respectively. Exo-Klenow fragment enzyme (Agilent Genomic DNA Enzymatic Labeling kit) was used to label these DNA.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent Oligo aCGH/ChIP-on-Chip Hybridization Kit) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially in wash buffer.
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
Images were quantified using Agilent Feature Extraction Software (v 10.5.1.1).
|
| Description |
TL9-S13133-65 kcal
Thymocytes
|
| Data processing |
Agilent Feature Extraction Software (v 10.5.1.1) was used for background subtraction and data extraction.
|
| |
|
| Submission date |
Jul 27, 2021 |
| Last update date |
Jan 06, 2023 |
| Contact name |
Masaaki Sunaoshi |
| E-mail(s) |
sunaoshi.masaaki@qst.go.jp
|
| Phone |
81-43-206-3160
|
| Organization name |
National Institutes for Quantum and Radiological Science and Technology
|
| Department |
Department of Radiation Effects Research, National Institute of Radiological Sciences
|
| Street address |
4-9-1 Anagawa, Inage-ku
|
| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19767 |
| Series (1) |
| GSE180952 |
Mouse thymic lymphomas arising after irradiation between with or without calorie restriction |
|
