|
| Status |
Public on Jul 19, 2010 |
| Title |
140x9_0-2h_embryos_total |
| Sample type |
SRA |
| |
|
| Source name |
embryos
|
| Organism |
Drosophila virilis |
| Characteristics |
tissue: embryos
strain: 140x9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was size selected (19-30nt) or small RNA-protein complexes were immunoprecipitated from extracts using specific polyclonal antibodies. These were then ligated to linkers at the 3' and 5' end using T4 RNA ligase, then PCR amplified and gel purified.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
19-30nt RNA isolated from 0-2h embryos of hybrids from crosses between strain 140 females and strain 9 males
processed_file_build: droVir3
|
| Data processing |
Sequenced reads were clipped to remove 3' adapter, collapsed to obtain multiplicity, then mapped to relevant genome with no mismatches. Annotations were made based on genomic co-ordinates after mapping. Mapping count refers to number of times a sequence is mapped to the genome.
|
| |
|
| Submission date |
Jun 01, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Nikolay V Rozhkov |
| E-mail(s) |
nik.rozhkov@gmail.com, rozhkov@cshl.edu
|
| Organization name |
CSHL
|
| Lab |
Hannon
|
| Street address |
1 Bungtown Rd.
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL10472 |
| Series (1) |
| GSE22067 |
Small RNA-based silencing strategies for transposons in the process of invading Drosophila species |
|
| Relations |
| SRA |
SRX023765 |
| BioSample |
SAMN00017198 |
