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| Status |
Public on Jul 19, 2010 |
| Title |
Small RNA-based silencing strategies for transposons in the process of invading Drosophila species |
| Organisms |
Drosophila melanogaster; Drosophila virilis |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We investigated a pool of small RNAs derived from transposon Penelope to probe the evolution of small RNA pathways in response to the transposon challenge in natural (D. virilis) and experimentally induced (D. melanogaster) colonization processes. In both species, Penelope was predominantly targeted by endo-siRNAs rather than by piRNAs. Although we observed correlations between Penelope transcription and dysgenesis, we could not correlate differences in maternally deposited Penelope piRNAs with the sterility of progeny. Instead, we found that strains, which produced dysgenic progeny, differed in their production of piRNAs from clusters in sub-telomeric regions, possibly indicating that changes in the overall piRNA repertoire underlie dysgenesis. Considered together, our data reveal unexpected plasticity in small RNA pathways in germ cells, both in the character of their responses to invading transposons and in the piRNA clusters that define their ability to respond to mobile elements.
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| Overall design |
Examination of total small RNA in 0-2h embryos, testes, ovaries and carcasses of several D. virilis strains and their hybrids; total small RNA and Ago2, Piwi, Aub and Ago3-associated small RNA in testes and ovaries of D. melanogaster.
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| Contributor(s) |
Rozhkov NV, Aravin AA, Zelentsova ES, Schostak NG, Sachidanandam R, McCombie WR, Hannon GJ, Evgen’ev MB |
| Citation(s) |
20581131, 21779346 |
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| Submission date |
Jun 01, 2010 |
| Last update date |
Aug 27, 2019 |
| Contact name |
Nikolay V Rozhkov |
| E-mail(s) |
nik.rozhkov@gmail.com, rozhkov@cshl.edu
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| Organization name |
CSHL
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| Lab |
Hannon
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| Street address |
1 Bungtown Rd.
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| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
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| Platforms (2) |
| GPL9058 |
Illumina Genome Analyzer (Drosophila melanogaster) |
| GPL10472 |
Illumina Genome Analyzer (Drosophila virilis) |
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| Samples (48)
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| Relations |
| SRA |
SRP002877 |
| BioProject |
PRJNA127489 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22067_NR_dmel_processed_file.txt.gz |
59.4 Mb |
(ftp)(http) |
TXT |
| GSE22067_NR_dvir_processed_file.txt.gz |
104.5 Mb |
(ftp)(http) |
TXT |
| GSE22067_readme.txt.gz |
290 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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