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Sample GSM549179 Query DataSets for GSM549179
Status Public on Dec 01, 2010
Title INCTC_Bone_marrow_normal_CD34+_Myeloid_12h
Sample type SAGE
Anchor NlaIII
Tag Count 62111
Tag Length 10
Source name Bone marrow CD34+ Hematopoietic Stem/Progenitor Cells cultured for 12 hours under myeloid differentiation stimulus
Organism Homo sapiens
Characteristics organ/tissue: bone marrow
tissue preparation: iliac crest aspiration
cell type: CD34+ HSPC cultured for 12 hours under myeloid stimulus
Biomaterial provider Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto - USP
Growth protocol For myeloid differentiation we used StemSpan (SteamCell Technologies, No. 09600) supplemented with 20% FBS, SCF (100ng/ml), Flt-3lig (50 ng/ml), IL-3 (20ng/ml), IL-6 (20 ng/ml) and GM-CSF (20ng/ml).
Extracted molecule total RNA
Extraction protocol TRIzol® Reagent
Description Bone marrow iliac crest aspirates from healthy adult donors. CD34+ cells were isolated to >95% purity by MACS Indirect CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec) following the manufacturer’s instructions. Cells were cultured for 12 hours under myeloid stimulus, following the growth protocol.
Data processing N/A
Submission date Jun 02, 2010
Last update date Jun 04, 2010
Contact name Daniel Guariz Pinheiro
Phone +55-16-2101-9300
Organization name Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
Department Departamento de Genética
Lab Laboratório de Genética Molecular e Bioinformática
Street address Rua Tenente Catão Roxo, 2501
City Ribeirão Preto
State/province São Paulo
ZIP/Postal code 14051-140
Country Brazil
Platform ID GPL4
Series (1)
GSE22092 Gene expression profiling of the early-stage CD34+ differentiation process

Data table header descriptions
TPM tags per million

Data table
CTGGGTTAAT 785 12638.66
TCAGATCTTT 760 12236.16
ATAATTCTTT 748 12042.96
GCATAATAGG 732 11785.35
GAGGGAGTTT 703 11318.45
TAGGTTGTCT 698 11237.94
TAATAAAGGT 624 10046.53
GAAAAATGGT 609 9805.03
GGGCTGGGGT 590 9499.12
GGATTTGGCC 514 8275.51
TGCACGTTTT 478 7695.90
TACCATCAAT 468 7534.90
AGGGCTTCCA 436 7019.69
TTGGTCCTCT 430 6923.09
TTCAATAAAA 419 6745.99
CCCATCGTCC 385 6198.58
CAATAAATGT 356 5731.67
TTGGGGTTTC 346 5570.67
CCTAGCTGGA 342 5506.27
TGTGTTGAGA 333 5361.37

Total number of rows: 17728

Table truncated, full table size 330 Kbytes.

Supplementary data files not provided

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