|
Status |
Public on Dec 01, 2010 |
Title |
INCTC_Bone_marrow_normal_CD34+_Myeloid_12h |
Sample type |
SAGE |
Anchor |
NlaIII
|
Tag Count |
62111 |
Tag Length |
10 |
|
|
Source name |
Bone marrow CD34+ Hematopoietic Stem/Progenitor Cells cultured for 12 hours under myeloid differentiation stimulus
|
Organism |
Homo sapiens |
Characteristics |
organ/tissue: bone marrow tissue preparation: iliac crest aspiration cell type: CD34+ HSPC cultured for 12 hours under myeloid stimulus
|
Biomaterial provider |
Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto - USP
|
Growth protocol |
For myeloid differentiation we used StemSpan (SteamCell Technologies, No. 09600) supplemented with 20% FBS, SCF (100ng/ml), Flt-3lig (50 ng/ml), IL-3 (20ng/ml), IL-6 (20 ng/ml) and GM-CSF (20ng/ml).
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIzol® Reagent
|
|
|
Description |
Bone marrow iliac crest aspirates from healthy adult donors. CD34+ cells were isolated to >95% purity by MACS Indirect CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec) following the manufacturer’s instructions. Cells were cultured for 12 hours under myeloid stimulus, following the growth protocol.
|
Data processing |
N/A
|
|
|
Submission date |
Jun 02, 2010 |
Last update date |
Jun 04, 2010 |
Contact name |
Daniel Guariz Pinheiro |
E-mail(s) |
dgpinheiro@usp.br
|
Phone |
+55-16-2101-9300
|
Organization name |
Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
|
Department |
Departamento de Genética
|
Lab |
Laboratório de Genética Molecular e Bioinformática
|
Street address |
Rua Tenente Catão Roxo, 2501
|
City |
Ribeirão Preto |
State/province |
São Paulo |
ZIP/Postal code |
14051-140 |
Country |
Brazil |
|
|
Platform ID |
GPL4 |
Series (1) |
GSE22092 |
Gene expression profiling of the early-stage CD34+ differentiation process |
|