|
| Status |
Public on Dec 01, 2010 |
| Title |
INCTC_Bone_marrow_normal_CD34+_Myeloid_12h |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
62111 |
| Tag Length |
10 |
| |
|
| Source name |
Bone marrow CD34+ Hematopoietic Stem/Progenitor Cells cultured for 12 hours under myeloid differentiation stimulus
|
| Organism |
Homo sapiens |
| Characteristics |
organ/tissue: bone marrow
tissue preparation: iliac crest aspiration
cell type: CD34+ HSPC cultured for 12 hours under myeloid stimulus
|
| Biomaterial provider |
Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto - USP
|
| Growth protocol |
For myeloid differentiation we used StemSpan (SteamCell Technologies, No. 09600) supplemented with 20% FBS, SCF (100ng/ml), Flt-3lig (50 ng/ml), IL-3 (20ng/ml), IL-6 (20 ng/ml) and GM-CSF (20ng/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol® Reagent
|
| |
|
| Description |
Bone marrow iliac crest aspirates from healthy adult donors. CD34+ cells were isolated to >95% purity by MACS Indirect CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec) following the manufacturer’s instructions. Cells were cultured for 12 hours under myeloid stimulus, following the growth protocol.
|
| Data processing |
N/A
|
| |
|
| Submission date |
Jun 02, 2010 |
| Last update date |
Jun 04, 2010 |
| Contact name |
Daniel Guariz Pinheiro |
| E-mail(s) |
dgpinheiro@usp.br
|
| Phone |
+55-16-2101-9300
|
| Organization name |
Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
|
| Department |
Departamento de Genética
|
| Lab |
Laboratório de Genética Molecular e Bioinformática
|
| Street address |
Rua Tenente Catão Roxo, 2501
|
| City |
Ribeirão Preto |
| State/province |
São Paulo |
| ZIP/Postal code |
14051-140 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL4 |
| Series (1) |
| GSE22092 |
Gene expression profiling of the early-stage CD34+ differentiation process |
|
