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Series GSE22092 Query DataSets for GSE22092
Status Public on Dec 01, 2010
Title Gene expression profiling of the early-stage CD34+ differentiation process
Organism Homo sapiens
Experiment type Expression profiling by SAGE
Summary To better understand the early events regulating lineage-specific hematopoietic differentiation, we analyzed the transcriptional profiles of CD34+ human hematopoietic stem and progenitor cells (HSPCs) subjected to differentiation stimulus.
Overall design CD34+ cells were cultured for 12 and 40 hours in liquid cultures with supplemented media favoring myeloid or erythroid commitment. Serial analysis of gene expression (SAGE) was employed to generate four independent libraries. CD34+ Hematopoietic Stem Progenitor Cells with no differentiation stimulus were used as a control library.
Contributor(s) Molfetta GA, Zanette DL, Pinheiro DG, Panepucci RA, Silva WA Jr, Zago MA
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Submission date Jun 02, 2010
Last update date Apr 18, 2012
Contact name Daniel Guariz Pinheiro
Phone +55-16-2101-9300
Organization name Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
Department Departamento de Genética
Lab Laboratório de Genética Molecular e Bioinformática
Street address Rua Tenente Catão Roxo, 2501
City Ribeirão Preto
State/province São Paulo
ZIP/Postal code 14051-140
Country Brazil
Platforms (1)
GPL4 SAGE:10:NlaIII:Homo sapiens
Samples (3)
GSM549178 INCTC_Bone_marrow_normal_CD34+
GSM549179 INCTC_Bone_marrow_normal_CD34+_Myeloid_12h
GSM549180 INCTC_Bone_marrow_normal_CD34+_Myeloid_40h
BioProject PRJNA128827

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