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Sample GSM549180 Query DataSets for GSM549180
Status Public on Dec 01, 2010
Title INCTC_Bone_marrow_normal_CD34+_Myeloid_40h
Sample type SAGE
Anchor NlaIII
Tag Count 63055
Tag Length 10
Source name Bone marrow CD34+ Hematopoietic Stem/Progenitor Cells cultured for 40 hours under myeloid differentiation stimulus
Organism Homo sapiens
Characteristics organ/tissue: bone marrow
tissue preparation: iliac crest aspiration
cell type: CD34+ HSPC cultured for 40 hours under myeloid stimulus
Biomaterial provider Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto - USP
Growth protocol For myeloid differentiation we used StemSpan (SteamCell Technologies, No. 09600) supplemented with 20% FBS, SCF (100ng/ml), Flt-3lig (50 ng/ml), IL-3 (20ng/ml), IL-6 (20 ng/ml) and GM-CSF (20ng/ml).
Extracted molecule total RNA
Extraction protocol TRIzol® Reagent
Description Bone marrow iliac crest aspirates from healthy adult donors. CD34+ cells were isolated to >95% purity by MACS Indirect CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec) following the manufacturer’s instructions. Cells were cultured for 40 hours under myeloid stimulus, following the growth protocol.
Data processing N/A
Submission date Jun 02, 2010
Last update date Jun 04, 2010
Contact name Daniel Guariz Pinheiro
Phone +55-16-2101-9300
Organization name Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
Department Departamento de Genética
Lab Laboratório de Genética Molecular e Bioinformática
Street address Rua Tenente Catão Roxo, 2501
City Ribeirão Preto
State/province São Paulo
ZIP/Postal code 14051-140
Country Brazil
Platform ID GPL4
Series (1)
GSE22092 Gene expression profiling of the early-stage CD34+ differentiation process

Data table header descriptions
TPM tags per million

Data table
GAGGGAGTTT 580 9198.32
GGGCTGGGGT 498 7897.87
GAAAAATGGT 466 7390.37
CTGGGTTAAT 465 7374.51
GGATTTGGCC 458 7263.50
TACCATCAAT 450 7136.63
TTGGTCCTCT 431 6835.30
CCCATCGTCC 414 6565.70
TGCACGTTTT 359 5693.44
ATAATTCTTT 337 5344.54
TCAGATCTTT 324 5138.37
CCTAGCTGGA 308 4884.62
GCATAATAGG 297 4710.17
TTCAATAAAA 276 4377.13
CACCTAATTG 275 4361.27
GAAGCAGGAC 272 4313.69
TAATAAAGGT 267 4234.40
GGCAAGCCCC 259 4107.53
AGGGCTTCCA 255 4044.09
TTCATACACC 238 3774.48

Total number of rows: 25835

Table truncated, full table size 481 Kbytes.

Supplementary data files not provided

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