|
| Status |
Public on Aug 16, 2021 |
| Title |
GC-Po-KdAr4 |
| Sample type |
SRA |
| |
|
| Source name |
ovary
|
| Organism |
Gallus gallus |
| Characteristics |
breed: White Leghorn
cell type: primary granulosa cells
sirna: AR-siRNA
|
| Treatment protocol |
The primary granulosa cells transfected with AR-specific siRNA (AR-siRNA) or NC nonsense siRNA (NC-siRNA) using Lipofectamine 3000 (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions, respectively.
|
| Growth protocol |
The primary granulosa cells were pre-cultured with Medium 199 (Gibco, USA) and 5% FBS (Gibco, USA) overnight (16h).
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted from primary granulosa cells using TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol.
The sequencing library was generated under the instruction manual with NEB Next UltraTM RNA Library Prep Kit for Illumina (NEB, USA). Obtained cDNA libraries were sequenced on an Illumina Nova platform and generated paired-end reads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
follicle
preovulatory
35-40 weeks
s20190308-GC-Po-KdAr4_FRAS190012313-1a_1
|
| Data processing |
Reads were filtered by removing adapters, low quality reads and unknown bases.
Clean reads were mapped to Gallus_gallus-5.0 of Ensembl using HISAT2 v2.1.0.
Genes expression levels were quantified by a software package: stringtie v1.3.4d.
Differentially expressed genes were calculated by Deseq2 v1.26.0.
Genome_build: Gallus_gallus-5.0
Supplementary_files_format_and_content: Tab-delimited gtf files include "cov", "FPKM", "TPM" values for each Sample, which generated by stringtie.
|
| |
|
| Submission date |
Aug 13, 2021 |
| Last update date |
Aug 17, 2021 |
| Contact name |
Ying Huang |
| E-mail(s) |
ying.huang@webmail.hzau.edu.cn
|
| Organization name |
Huazhong Agricutural University
|
| Street address |
No.1,Shizishan Street · Hongshan District · Wuhan · Hubei Province
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL26853 |
| Series (2) |
| GSE163891 |
Comparative analysis among different species reveals that the androgen receptor regulates chicken follicle selection through species-specific genes related to follicle development |
| GSE182083 |
Comparative analysis among different species reveals that androgen receptor is a core transcriptional factor regulating the chicken follicular selection and ovulation [RNA-Seq 2] |
|
| Relations |
| BioSample |
SAMN20773804 |
| SRA |
SRX11745813 |
