|
| Status |
Public on Jun 10, 2010 |
| Title |
Rat liver TCDD_52 week_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
sprague dawley rat liver (52wks)
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: sprague dawley
tissue: liver
agent: 100ng TCDD
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the Qiagen RNeasy kit following the manufacture's instructions.
|
| Label |
biotin
|
| Label protocol |
High quality RNA was transformed into biotinylated cRNA by the Roswell Park Cancer Institute Gene Expression Facility (Buffalo, NY)
|
| |
|
| Hybridization protocol |
cRNA was hybridized to RGU34A GeneChips (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
GeneChips were scanned with the Affymetrix 428 scanner.
|
| Data processing |
Probe-level data from cell intensity files were background subtracted and normalized by the gc-Robust Multiarray Analysis method using ArrayAssist.
|
| |
|
| Submission date |
Jun 09, 2010 |
| Last update date |
Jun 09, 2010 |
| Contact name |
Corie Ellison |
| Organization name |
University at Buffalo
|
| Department |
Pharmacology and Toxicology
|
| Lab |
James Olson
|
| Street address |
102 Farber hall
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14214 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE22263 |
Effect of 52 weeks of chronic exposure to TCDD AND PCB126 on hepatic gene expression |
|
