|
Status |
Public on Jun 10, 2010 |
Title |
Rat liver_300ng PCB126_52 week_ rep1 |
Sample type |
RNA |
|
|
Source name |
sprague dawley rat liver (52wks)
|
Organism |
Rattus norvegicus |
Characteristics |
strain: sprague dawley tissue: liver agent: 300ng PCB126
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with the Qiagen RNeasy kit following the manufacture's instructions.
|
Label |
biotin
|
Label protocol |
High quality RNA was transformed into biotinylated cRNA by the Roswell Park Cancer Institute Gene Expression Facility (Buffalo, NY)
|
|
|
Hybridization protocol |
cRNA was hybridized to RGU34A GeneChips (Affymetrix, Santa Clara, CA)
|
Scan protocol |
GeneChips were scanned with the Affymetrix 428 scanner.
|
Data processing |
Probe-level data from cell intensity files were background subtracted and normalized by the gc-Robust Multiarray Analysis method using ArrayAssist.
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|
|
Submission date |
Jun 09, 2010 |
Last update date |
Jun 09, 2010 |
Contact name |
Corie Ellison |
Organization name |
University at Buffalo
|
Department |
Pharmacology and Toxicology
|
Lab |
James Olson
|
Street address |
102 Farber hall
|
City |
Buffalo |
State/province |
NY |
ZIP/Postal code |
14214 |
Country |
USA |
|
|
Platform ID |
GPL85 |
Series (1) |
GSE22263 |
Effect of 52 weeks of chronic exposure to TCDD AND PCB126 on hepatic gene expression |
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