|
| Status |
Public on Jul 17, 2022 |
| Title |
hTS-siYT-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human trophoblast stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell ytpe: Human trophoblast stem cells
sirna: YAP/TAZ
|
| Treatment protocol |
Cells were transfected with YT- (10 nM ea), TEAD (20nM), or NC- (20 nM) siRNA, and cells were further incubated for 72 h in the TS medium.
|
| Growth protocol |
Human trophoblast stem cells were cultured in TS medium (DMEM/F12 supplemented with 0.1 mM 2-mercaptoethanol, 0.2% FBS, 1% penicillin/streptomycin, 0.3% bovine serum albumin, 1% ITS-X supplement , 1.5 μg/mL l-ascorbic acid, 50 ng/mL EGF, 2 μM CHIR99021, 0.5 μM A83–01, 1 μM SB431542, 0.8 mM valproic acid, and 5 μM Y27632) in a plate pre-coated with 1 μg/ml Col IV.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated from the cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific) with DNase I treatment, followed by RNA integrity number (RIN) measured using the Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
RNA samples were labeled to the Clariom S Human Array according to standardized protocols from Affymetrix..
|
| |
|
| Hybridization protocol |
RNA samples were hybridized to Human Clariom S Gene Chip according to standardized protocols from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
RMA expression value derived from Expression Console software
|
| Data processing |
Raw gene expression data was normalized using RMA in Affymetrix Expression Console.
|
| |
|
| Submission date |
Aug 27, 2021 |
| Last update date |
Jul 17, 2022 |
| Contact name |
Tetsuya Mizutani |
| E-mail(s) |
mizutani237@gmail.com
|
| Organization name |
Fukui Prefectural University
|
| Street address |
4-1-1 Kenjojima
|
| City |
Eiheiji |
| State/province |
Fukui |
| ZIP/Postal code |
910-1195 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE182900 |
Role of YAP/TAZ-TEAD in human trophoblast |
|
