|
| Status |
Public on Oct 01, 2010 |
| Title |
left ventricle, aorta banding, cxcr5 knockout, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
left ventricle, aorta banding, cxcr5 knockout
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
genotype: CXCR5 -/-, C57BL/6 background
tissue: left ventricle myocardium
|
| Treatment protocol |
Mice were anesthetized with isoflurane and sacrificed by cervical dislocation. Left ventricle was quickly excised and immediately frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with an RNeasy mini kit according to the manufacturer's instructions.
|
| Label |
phycoerythrin
|
| Label protocol |
According to standard Affymetrix protocol (FS450_00007).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 h at 450C in the hybridization oven at 60 rpm, as described by manufacturer (Affymetrix).
|
| Scan protocol |
Gene Chips were scanned using the Hewlett Packard GeneArray Scanner 3000 7G.
|
| Data processing |
The data were preprocessed using the robust multi-array average (RMA) package in R with default settings, i.e. quantile normalization and RMA background correction
|
| |
|
| Submission date |
Jun 10, 2010 |
| Last update date |
Aug 04, 2010 |
| Contact name |
Ståle Nygård |
| E-mail(s) |
stale.nygard@medisin.uio.no
|
| Phone |
+47 95129127
|
| Organization name |
University of Oslo
|
| Street address |
Institute for Experimental Medical Research, Oslo University Hospital - Ulleval
|
| City |
Oslo |
| ZIP/Postal code |
0407 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22295 |
Lack of chemokine signaling through CXCR5 causes mortality, ventricular dilatation and deranged matrix during pressure overload |
|
