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Status |
Public on Oct 01, 2010 |
Title |
left ventricle, aorta banding, wildtype, rep3 |
Sample type |
RNA |
|
|
Source name |
left ventricle, aorta banding, wildtype
|
Organism |
Mus musculus |
Characteristics |
gender: male genotype: Wildtype C57BL/6 tissue: left ventricle myocardium
|
Treatment protocol |
Mice were anesthetized with isoflurane and sacrificed by cervical dislocation. Left ventricle was quickly excised and immediately frozen in liquid nitrogen.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with an RNeasy mini kit according to the manufacturer's instructions.
|
Label |
phycoerythrin
|
Label protocol |
According to standard Affymetrix protocol (FS450_00007).
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|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 h at 450C in the hybridization oven at 60 rpm, as described by manufacturer (Affymetrix).
|
Scan protocol |
Gene Chips were scanned using the Hewlett Packard GeneArray Scanner 3000 7G.
|
Data processing |
The data were preprocessed using the robust multi-array average (RMA) package in R with default settings, i.e. quantile normalization and RMA background correction
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|
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Submission date |
Jun 10, 2010 |
Last update date |
Aug 04, 2010 |
Contact name |
Ståle Nygård |
E-mail(s) |
stale.nygard@medisin.uio.no
|
Phone |
+47 95129127
|
Organization name |
University of Oslo
|
Street address |
Institute for Experimental Medical Research, Oslo University Hospital - Ulleval
|
City |
Oslo |
ZIP/Postal code |
0407 |
Country |
Norway |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE22295 |
Lack of chemokine signaling through CXCR5 causes mortality, ventricular dilatation and deranged matrix during pressure overload |
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