|
| Status |
Public on Oct 27, 2021 |
| Title |
SAMPLE 7: wt1 30min |
| Sample type |
RNA |
| |
|
| Source name |
wt culture 1; 30 min after Zn treatment
|
| Organism |
Pseudomonas aeruginosa PAO1 |
| Characteristics |
strain: PAO1 WT Laboratory strain
treatment: zinc induction
timepoint: 30min
|
| Growth protocol |
Cultures were grown at 37°C in modified Luria-Broth medium as described in DOI: 10.3389/fmicb.2021.739988
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each time point, 0,5ml of culture was mixed with 1ml of RNA protect Reagent. Total RNAs were then extracted with RNeasy mini kit (Qiagen) according to the manufacturer'sinstructions and treated withRQ+ RNAse-free DNAse for 2h at 37°C, followd by phenol/chloroform extraction and ethanol precipitation. 50ng of total RNA were used for NanoString nCounter expression analysis.
|
| Label |
direct hybridization with molecular fluorecent barcode probes
|
| Label protocol |
no labelling - Direct hybridization of the RNA with the nanostring codeset (mix of probes)
|
| |
|
| Hybridization protocol |
50 ng of RNA were hybridized at 65°C over night
|
| Scan protocol |
Hybridization mixes were then loaded in the nCounter Prep Station for purification of complexes and loading on the cartridge. Cartridges were scanned on the nCounter Flex scanner.
|
| Data processing |
according to J Microbiol Methods. 2011 Feb;84(2):327-34
|
| |
|
| Submission date |
Aug 30, 2021 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Natacha Civic |
| E-mail(s) |
natacha.civic@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
iGE3 Genomics Platform
|
| Lab |
University of Geneva
|
| Street address |
Rue Michel-Servet 1
|
| City |
Geneva |
| ZIP/Postal code |
1211-4 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL30568 |
| Series (1) |
| GSE183060 |
Global analysis of the zinc homeostasis network in Pseudomonas aeruginosa (DOI: 10.3389/fmicb.2021.739988) |
|
