|
| Status |
Public on Jun 12, 2010 |
| Title |
Tomato Heat Susceptible Treated rep-2 |
| Sample type |
RNA |
| |
|
| Source name |
Leave at flowering stage at High temperature (40C) stress condition
|
| Organism |
Solanum lycopersicum |
| Characteristics |
genotype: Heat Susceptible Genotype
|
| Treatment protocol |
Sample 1,2,3,7,8,9,13,14,15,19,20,21 were grown in normal growth conditions without any stress in green house. While sample 4,5,6, and 10,11,12 were subjected to water stress by with holding water for 7 days. Sample 16,17,18 and 22,23,24 were subjected to 40C for 60 min in growth chamber befor sample collection.
|
| Growth protocol |
Plants were sown in plastic pots at optimal conditions for tomato plants.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Tomato Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Description |
SAMPLE 17
Gene expression data from Tomato Heat Susceptible treated plants
|
| Data processing |
The data were analyzed with Affymetrix Expression Console and RMA version 1.0 as normalization method.
|
| |
|
| Submission date |
Jun 11, 2010 |
| Last update date |
Jun 11, 2010 |
| Contact name |
Ashutosh Rai |
| E-mail(s) |
ashutoshraiiivr@gmail.com
|
| Phone |
+919451959351
|
| Organization name |
Indian Institute of Vegetable Research
|
| Department |
Crop Improvment
|
| Lab |
Functional Genomics
|
| Street address |
Post Box-1. Jakhini Shahanshapur
|
| City |
Varanasi |
| State/province |
Uttar Pradesh |
| ZIP/Postal code |
221305 |
| Country |
India |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE22304 |
Expression data in response to abiotic stresses in tomato at flowering stage |
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