|
| Status |
Public on Oct 13, 2010 |
| Title |
colon of mouse treated with DSS for 2 day, D2M52 |
| Sample type |
RNA |
| |
|
| Source name |
colon
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
time point (day): 2
agent: 3% Dextran Sodium Sulphate
|
| Treatment protocol |
A 3% Dextran Sodium Sulphate solution was administered in the drinking water for the mice.
|
| Growth protocol |
C57BL/6J mice aged 12-14 weeks old were housed according to institutional IACUC regulations and the National Research Council's Guide for Care and Use of Laboratory Animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by using Qiagen RNA easy kits according the manufacture's instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 500 as normalization method.
|
| |
|
| Submission date |
Jun 11, 2010 |
| Last update date |
Oct 13, 2010 |
| Contact name |
Kevil G Christopher |
| E-mail(s) |
ckevil@lsuhsc.edu
|
| Phone |
3186754694
|
| Organization name |
Louisiana State University Health Science Center, Shreveport
|
| Department |
Pathology
|
| Street address |
1501 Kings Highway
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE22307 |
Expression data from mouse colon tissue response to DSS induction at day 0, 2, 4 and 6 |
|
