|
| Status |
Public on Sep 23, 2022 |
| Title |
fasting_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
liver,fasting
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
gender: male
|
| Treatment protocol |
Fasting group were fast overnight about 14-16h, feeding group were given a nutrition ingestion after fast.
|
| Growth protocol |
All the mice were housed in a specific-pathogen-free (SPF) environment with controlled conditions, a temperature of 23±3°C and a humidity of 35±5% under a12-h dark/light cycle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by using Trizol reagent from liver
|
| Label |
Cy3
|
| Label protocol |
Agilent Quick Amp Labeling Kit was used for sample labeling.
|
| |
|
| Hybridization protocol |
Hybridization was performed in Agilent’s SureHyb Hybridization Chambers.
|
| Scan protocol |
After washing, slides were scanned with the Agilent DNA Microarray Scanner.
|
| Description |
Gene expression after 16hr fasting in mouse liver
|
| Data processing |
Data was extracted using Agilent Feature Extraction software. Normalization was performed using Agilent GeneSpring GX 11.5.
Raw signal intensities were normalized in quantile method by GeneSpring GX v11.5, and low intensity genes were filtered.
|
| |
|
| Submission date |
Sep 13, 2021 |
| Last update date |
Sep 23, 2022 |
| Contact name |
ying yan |
| E-mail(s) |
yanying2017@sibs.ac.cn
|
| Organization name |
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences
|
| Street address |
320 Yueyang Road, Shanghai
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE184055 |
Hepatic thyroid hormone signalling modulates glucose homeostasis through the regulation of GLP-1 production via bile acid-mediated FXR antagonism |
|
