|
| Status |
Public on Dec 29, 2010 |
| Title |
Mutant 2 |
| Sample type |
RNA |
| |
|
| Source name |
mutant leaves, 2 months old
|
| Organism |
Medicago truncatula |
| Characteristics |
genotype: CYP716A12 mutation
age: 2 months
tissue: leaves
|
| Growth protocol |
CYP716A12 mutant and wild-type R108 plants were grown in a growth chamber under 16 hours daylight at 22C and 8 hours darkness at 18C. Plants were grown in 12cm pots containing a mixture 9:1 vol/vol of a sandy loam soil and laterlite sand.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeay mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled and fragmented using the Affymetrix IVT express kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Medicago Genome Arrays (Affymetrix) at the NASC Microarray Core Facility according to manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned at the NASC Microarray Core Facility according to the manufacturer's instructions (Affymetrix).
|
| Description |
2_E2_Medicago
|
| Data processing |
Data analysis and statistical analysis was performed using the GeneSpring GX 11 programm from Agilent Technologies. Raw data was pre-normalised using the RMA algorithm.
|
| |
|
| Submission date |
Jul 08, 2010 |
| Last update date |
Dec 29, 2010 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE22835 |
Characterization of a Medicago truncatula P450 monooxygenase mutant |
|
