|
| Status |
Public on Apr 03, 2023 |
| Title |
pd-JAO-dc_Hypoxia [pt#10] |
| Sample type |
RNA |
| |
|
| Source name |
primary pd-JAO derived cells under hypoxia condition
|
| Organism |
Homo sapiens |
| Characteristics |
subject source id: Patient JMML#10
cell type: primary JMML pd-JAO derived cells
treatment: hypoxia treatment
|
| Treatment protocol |
Normoxia (20% of oxygen level); Hypoxia (<2% of oxygen level)
|
| Growth protocol |
JMML cells were cultured in RPMI 1640 Colture Medium supplemented with 10% BIT9500 Serum Substitute, 2mM L-Glutamine and 0.5% Penicillin/Streptomycin antibiotics and SCF (50ng/mL), TPO (50ng/mL), FLT-3L (50ng/mL), IL-3 (20ng/mL) and IL-6 (20ng/mL) cytokines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using MiRNeasy Micro Kit (QIAGEN, Hilden, DE)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 5 to 50ng of Total RNA using the Affymetrix 3’IVT Pico Kit and 12 PCR cycles
|
| |
|
| Hybridization protocol |
6.6 ug were fragmented; following fragmentation,20 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org] using RMA
|
| |
|
| Submission date |
Nov 11, 2021 |
| Last update date |
Apr 03, 2023 |
| Contact name |
silvia bresolin |
| E-mail(s) |
silvia.bresolin@unipd.it
|
| Phone |
+390498215487
|
| Organization name |
università di padova
|
| Department |
department of women's and children's health
|
| Lab |
SSD ematologia-clinica sperimentale
|
| Street address |
via giustiniani 3
|
| City |
padova |
| State/province |
padova |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE188608 |
Long-term proliferation of immature hypoxia-dependent JMML cells supported by a 3D in vitro system |
|
