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Status |
Public on Apr 03, 2023 |
Title |
pd-JAO-dc_Hypoxia [pt#3] |
Sample type |
RNA |
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Source name |
primary pd-JAO derived cells under hypoxia condition
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Organism |
Homo sapiens |
Characteristics |
subject source id: Patient JMML#3 cell type: primary JMML pd-JAO derived cells treatment: hypoxia treatment
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Treatment protocol |
Normoxia (20% of oxygen level); Hypoxia (<2% of oxygen level)
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Growth protocol |
JMML cells were cultured in RPMI 1640 Colture Medium supplemented with 10% BIT9500 Serum Substitute, 2mM L-Glutamine and 0.5% Penicillin/Streptomycin antibiotics and SCF (50ng/mL), TPO (50ng/mL), FLT-3L (50ng/mL), IL-3 (20ng/mL) and IL-6 (20ng/mL) cytokines.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using MiRNeasy Micro Kit (QIAGEN, Hilden, DE)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 5 to 50ng of Total RNA using the Affymetrix 3’IVT Pico Kit and 12 PCR cycles
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Hybridization protocol |
6.6 ug were fragmented; following fragmentation,20 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Scan GCS3000.
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Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org] using RMA
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Submission date |
Nov 11, 2021 |
Last update date |
Apr 03, 2023 |
Contact name |
silvia bresolin |
E-mail(s) |
silvia.bresolin@unipd.it
|
Phone |
+390498215487
|
Organization name |
università di padova
|
Department |
department of women's and children's health
|
Lab |
SSD ematologia-clinica sperimentale
|
Street address |
via giustiniani 3
|
City |
padova |
State/province |
padova |
ZIP/Postal code |
35128 |
Country |
Italy |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE188608 |
Long-term proliferation of immature hypoxia-dependent JMML cells supported by a 3D in vitro system |
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