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| Status |
Public on Mar 22, 2022 |
| Title |
NC_EBL_rep3 |
| Sample type |
SRA |
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| Source name |
embryo
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| Organism |
Bos taurus |
| Characteristics |
developmental stage: early blastocyst
age: 7.0 day after fertilization
treatment: embryos were injected with BE3 mRNA at zygote stage
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Smart-seq2
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Reads were trimmed by Trimmomatic to remove the bases with low quality and adapter sequences
The trimmed reads were align to ARS-UCD1.2 by Hisat2
The raw reads counts of all expressed genes were quatified by FeatureCounts
The abundance of all expressed genes were normalized by DEseq2
Genome_build: ARS-UCD1.2
Supplementary_files_format_and_content: text files include normalized reads counts calculated by DESeq2 for each sample
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| Submission date |
Nov 22, 2021 |
| Last update date |
Mar 22, 2022 |
| Contact name |
Kun Zhang |
| E-mail(s) |
kzhang@zju.edu.cn
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| Organization name |
Zhejiang University
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| Street address |
866 Yuhangtang Rd
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| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platform ID |
GPL26012 |
| Series (1) |
| GSE189318 |
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency |
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| Relations |
| BioSample |
SAMN23383912 |
| SRA |
SRX13191024 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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