|
| Status |
Public on Mar 22, 2022 |
| Title |
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Proper lineage specification is extremely important for the embryo's implantation and development. Numerous findings of lineage specification emerged in the mouse embryo but remain unclear in the bovine embryo due to the limited techniques. Here, our study is the first to demonstrate that the single base editor (ABE7.10 and BE3) can be applied to bovine embryos for base conversion, which contributes us to further exploring the lineage specification of the bovine embryo. We further show SOX2 is not necessary for blastocyst formation but is required for ICM maintenance in bovine early embryo development. The underlying mechanism may be that SOX2 promoted the differentiation of ICM by maintaining the expression levels of OCT4 and NANOG. Meanwhile, the disappearance of SOX2 in TE of bovine late blastocyst depended on the expression of CDX2.
|
| |
|
| Overall design |
PolyA RNA profiles of mouse and bovine embryos
|
| |
|
| Contributor(s) |
Zhang K, Luo L, Shi Y, Dang Y |
| Citation(s) |
35788719 |
| Submission date |
Nov 22, 2021 |
| Last update date |
Jul 25, 2022 |
| Contact name |
Kun Zhang |
| E-mail(s) |
kzhang@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Rd
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA782550 |
Less...
More...