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Status |
Public on Mar 22, 2022 |
Title |
SOX2KO_EBL_rep5 |
Sample type |
SRA |
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Source name |
embryo
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Organism |
Bos taurus |
Characteristics |
developmental stage: early blastocyst age: 7.0 day after fertilization treatment: embryos were injected with BE3 mRNA and SOX2 sgRNAs mixture at zygote stage
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Extracted molecule |
polyA RNA |
Extraction protocol |
Smart-seq2
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
Reads were trimmed by Trimmomatic to remove the bases with low quality and adapter sequences The trimmed reads were align to ARS-UCD1.2 by Hisat2 The raw reads counts of all expressed genes were quatified by FeatureCounts The abundance of all expressed genes were normalized by DEseq2 Genome_build: ARS-UCD1.2 Supplementary_files_format_and_content: text files include normalized reads counts calculated by DESeq2 for each sample
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Submission date |
Nov 22, 2021 |
Last update date |
Mar 22, 2022 |
Contact name |
Kun Zhang |
E-mail(s) |
kzhang@zju.edu.cn
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Organization name |
Zhejiang University
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Street address |
866 Yuhangtang Rd
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City |
Hangzhou |
ZIP/Postal code |
310058 |
Country |
China |
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Platform ID |
GPL26012 |
Series (1) |
GSE189318 |
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency |
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Relations |
BioSample |
SAMN23383906 |
SRA |
SRX13191030 |
Supplementary data files not provided |
SRA Run Selector![Help](/coreweb/images/long_help4.gif) |
Raw data are available in SRA |
Processed data are available on Series record |
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