|
| Status |
Public on Nov 27, 2023 |
| Title |
iWAT aKO_2 |
| Sample type |
RNA |
| |
|
| Source name |
inguinal white adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: inguinal white adipose tissue
strain: Senp2flox/flox ; Adipoq Cre
|
| Treatment protocol |
Eight week-old mice were fed a high-fat diet, (HFD; 58 kcal% fat w/sucrose, D12331; Research Diets, New Brunswick, NJ, USA) for 12 weeks.
|
| Growth protocol |
All animals were housed at 22-24°C, with a 12:12-h light-dark cycle and ad libitum access to diet and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using TRIzol (Invitrogen, MA, USA) according to the manufacturer’s instruction.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA was prepared from 0.5 ug total RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNeasy column purification (QIAGEN, Valencia, CA).
|
| |
|
| Hybridization protocol |
Cy3 labeled cDNA was directly mix with hybridization buffer and Agilent blocking agent. Then, it was hybridized on microarrays for 40h at 65ºC in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent).
|
| Scan protocol |
The array was scanned using a SureScan Microarray Scanner (Agilent).
|
| Description |
Gene expression profiles of iWAT obtained from Senp2-aKO mouse
|
| Data processing |
The intensity data of each individual hybridization were extracted and the quality was assessed with the Feature Extraction software (version 11.5.1) (Agilent). The log2-intensities of the probes from the arrays were normalized using the quantile normalization method.
|
| |
|
| Submission date |
Nov 22, 2021 |
| Last update date |
Nov 27, 2023 |
| Contact name |
Sehyun Chae |
| E-mail(s) |
shchae@kbri.re.kr
|
| Organization name |
Korea Brain Research Institute
|
| Department |
Neurovascular Unit Research Group
|
| Street address |
61, Cheomdan-ro, Dong-gu
|
| City |
Daegu |
| ZIP/Postal code |
41062 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE189326 |
SENP2 suppresses browning of white adipose tissues by de-conjugating SUMO from C/EBPβ |
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