|
| Status |
Public on Nov 03, 2022 |
| Title |
Raptor-ShhCre Mutant rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic lung
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Raptorf/f; ShhCre/+
tissue: Mouse embryonic lung
Stage: E11.5dpc
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Embronic lungs were collected, snap freezed in liquid nitrogen, and RNAs were extracted with the RNeasy Mini Kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
Mutant lung
|
| Data processing |
remove adaptor with 'trim_galore'
align the reading to genome with 'Hisat2'
sort the SAM file and convert it to BAM file
create table counts with 'featurecounts'
generate DEG table with DeSeq2 in R
Genome_build: mm10
Supplementary_files_format_and_content: csv file
|
| |
|
| Submission date |
Nov 22, 2021 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Pao-Tien Chuang |
| Organization name |
University of California, San Francisco
|
| Street address |
555 Mission Bay Blvd. South
|
| City |
San Francisco |
| ZIP/Postal code |
CA 94158-3118 |
| Country |
USA |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE189327 |
mTORC1 signaling facilitates differential stem cell differentiation to shape the developing murine lung and is associated with mitochondrial capacity |
|
| Relations |
| BioSample |
SAMN23384329 |
| SRA |
SRX13191391 |
