|
| Status |
Public on Dec 17, 2021 |
| Title |
Laos2 |
| Sample type |
genomic |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
population: Lao
region: Southeast Asia
tissue: blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
|
| Label |
Cy3, Cy5
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48°C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol, dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
| Description |
Genomic DNA extracted from blood was genotyped using HumanOmniExpress 24 v1.1 BeadChip (Illumina).
|
| Data processing |
Image data was analyzed using Genomestudio v2.0 with GT module 2.0.4 (Illumina).
Genotype: AA,AB,BB,or NC (no call)
|
| |
|
| Submission date |
Dec 15, 2021 |
| Last update date |
Dec 17, 2021 |
| Contact name |
Tuuli Reisberg |
| Organization name |
University of Tartu
|
| Street address |
Riia 23
|
| City |
Tartu |
| ZIP/Postal code |
51010 |
| Country |
Estonia |
| |
|
| Platform ID |
GPL22678 |
| Series (1) |
| GSE190958 |
On the genetic constitution of the Marquesas |
|
