|
Status |
Public on Dec 12, 2022 |
Title |
Early-onset B-cell lymphoma case X1 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Early-onset precursor B-cell lymphoma by the irradiation of X-rays at 1 week of age
|
Organism |
Mus musculus |
Characteristics |
strain: B6C3F1 gender: Male treatment: irradiation of X-rays at 1 week of age disease state: Early-onset precursor B-cell lymphoma tissue: B-cell lymphoma
|
Treatment protocol |
Mice were left untreated or were subjected to whole-body irradiated with gamma-rays (4 Gy) or X-rays (3.8 Gy) at 1 or 7 weeks of age.
|
Growth protocol |
Mice were housed in autoclaved cages and maintained in a room with a controlled temperature (23 ± 3°C) and humidity (50 ± 10%) under a regular 12-hours light, 12-hours dark cycle. They were fed a standard laboratory animal diet and sterilized water ad libitum.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA of B-cell lymphomas and normal normal ears were extarcted by AllPrep DNA/RNA/Protein Mini Kit and DNeasy Blood & Tissue Kit (Qiagen), respectively.
|
Label |
Cy5
|
Label protocol |
Labeling reactions with cyanine 5-UTP and cyanine 3-UTP were performed according to the Agilent SureTag DNA labeling kit (Agilent Technologies, Santa Clara, CA, USA).
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|
|
Channel 2 |
Source name |
Normal ear
|
Organism |
Mus musculus |
Characteristics |
strain: B6C3F1 gender: Male tissue: Ear skin
|
Treatment protocol |
Mice were left untreated or were subjected to whole-body irradiated with gamma-rays (4 Gy) or X-rays (3.8 Gy) at 1 or 7 weeks of age.
|
Growth protocol |
Mice were housed in autoclaved cages and maintained in a room with a controlled temperature (23 ± 3°C) and humidity (50 ± 10%) under a regular 12-hours light, 12-hours dark cycle. They were fed a standard laboratory animal diet and sterilized water ad libitum.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA of B-cell lymphomas and normal normal ears were extarcted by AllPrep DNA/RNA/Protein Mini Kit and DNeasy Blood & Tissue Kit (Qiagen), respectively.
|
Label |
Cy3
|
Label protocol |
Labeling reactions with cyanine 5-UTP and cyanine 3-UTP were performed according to the Agilent SureTag DNA labeling kit (Agilent Technologies, Santa Clara, CA, USA).
|
|
|
|
Hybridization protocol |
Slides were hybridised in 1X Agilent Blocking Agent/Hi-RPM Buffer for 24 hours at 67°C in a rotating hybridization oven, then washed with the Agilent Oligo aCGH Buffer 1 and Buffer 2 in an ozone-depleted hood.
|
Scan protocol |
Scanned on an Agilent microarray scanner.
|
Description |
Early-pBLX1
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
|
|
Submission date |
Dec 22, 2021 |
Last update date |
Dec 14, 2022 |
Contact name |
Kazuhiro DAINO |
E-mail(s) |
daino.kazuhiro@qst.go.jp
|
Organization name |
National Institutes for Quantum and Radiological Science and Technology
|
Street address |
4-9-1 Anagawa, Inage-ku
|
City |
Chiba |
ZIP/Postal code |
263-8555 |
Country |
Japan |
|
|
Platform ID |
GPL19767 |
Series (1) |
GSE192455 |
Genomic DNA copy number alterations of mouse B-cell lymphomas induced by ionizing radiation |
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