|
| Status |
Public on Dec 06, 2023 |
| Title |
Mice skin tissue-Vehicle5 |
| Sample type |
SRA |
| |
|
| Source name |
skin tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: dorsal skin
strain: C3H
treatment: Vehicle of TDM10842
|
| Treatment protocol |
Except Blank group, the Vehicle and TDM group were accepted twice application of solvents and drugs (0, 24h) with 60ul once a time.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Vehicle5
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Genome_build: mm9
Supplementary_files_format_and_content: Matrix table with raw gene counts and fpkm for every gene and every sample
|
| |
|
| Submission date |
Dec 30, 2021 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Peiqiu Zhu |
| E-mail(s) |
1911110289@bjmu.edu.cn
|
| Phone |
86-18801229335
|
| Organization name |
Peking University First Hospital
|
| Department |
Dermatology
|
| Street address |
Xishiku Street 8
|
| City |
Beijing |
| ZIP/Postal code |
100034 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE192797 |
Thyroid hormone receptor agonist promotes hair growth in mice |
|
| Relations |
| BioSample |
SAMN24518979 |
| SRA |
SRX13556961 |
