|
| Status |
Public on Oct 19, 2010 |
| Title |
Wild Type MEFs; biological replicate 3; control for Snf5 Ezh2-deficient MEFs |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
age: E13.5
genotype: Wild Type
cell type: embryonic fibroblasts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from MEF and Lymphoma samples using RNA Trizol per the manufacturer’s protocol and Qiagen RNAeasy purification. RNA was hybridized to Affymetrix Mouse 430A 2.0 arrays, and CEL files were preprocessed using Robust Multichip Average (RMA).
|
| Label |
biotin
|
| Label protocol |
RNA of interest is converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA.
|
| |
|
| Hybridization protocol |
The cRNA is hybridized to a microarray chip. The bound cRNA is stained with fluorescent streptavidin.
|
| Scan protocol |
The microarray chip is then scanned using a laser, and the positions and intensities of the fluorescent emissions are captured. These measurements provide the basis of subsequent biostatistical analysis.
|
| Description |
Gene expression data from WT MEFs
|
| Data processing |
Data was analyzed using the Gene Pattern software. RMA normalization.
|
| |
|
| Submission date |
Aug 16, 2010 |
| Last update date |
Oct 19, 2010 |
| Contact name |
Boris G. Wilson |
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Pediatric Oncology
|
| Lab |
Charles Roberts
|
| Street address |
44 Binney Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE23656 |
Epigenetic antagonism between Snf5 and Ezh2 during oncogenic transformation |
| GSE23659 |
Epigenetic antagonism between Snf5 and Ezh2 during oncogenic transformation and elevated levels of H3K27me3 in Snf5-deficient cells |
|
| Relations |
| Reanalysis of |
GSM580394 |
