NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE23656 Query DataSets for GSE23656
Status Public on Oct 19, 2010
Title Epigenetic antagonism between Snf5 and Ezh2 during oncogenic transformation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Epigenetic alterations have been increasingly implicated in oncogenesis. Analysis of Drosophila mutants suggests that Polycomb and SWI/SNF complexes can serve antagonistic developmental roles. However, the relevance of this relationship to human disease is unclear. Here we have investigated functional relationships between these epigenetic regulators in oncogenic transformation. Mechanistically, we show that loss of the SNF5 tumor suppressor leads to elevated expression of the Polycomb gene EZH2 and that Polycomb targets are broadly H3K27-trimethylated and repressed in SNF5-deficient fibroblasts and cancers. Further, we show antagonism between SNF5 and EZH2 in the regulation of stem cell-associated programs and that Snf5 loss activates those programs. Finally, using conditional mouse models, we show that inactivation of Ezh2 blocks tumor formation driven by Snf5 loss.
 
Overall design Mouse Embryonic Fibroblasts (MEFs) conditionally inactivated for Ezh2, Snf5 and Ezh2, or from control WT MEFs were used to evaluated epigenetic antagonism between Snf5 and Ezh2 in the control of gene expression programs. Snf5-deficient lymphoma samples and control CD8+ WT T-cells were used to evaluate genetic programs misregulated by Snf5 inactivation during tumorigenesis. RNA was isolated from each of these samples and used for gene expression profiling on Affymetrix arrays.
 
Contributor(s) Roberts C, Wilson B
Citation(s) 20951942, 21926465
Submission date Aug 16, 2010
Last update date Aug 27, 2019
Contact name Boris G. Wilson
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Charles Roberts
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (23)
GSM580392 Wild Type MEFs; biological replicate 1; control for Ezh2-deficient MEFs
GSM580393 Wild Type MEFs; biological replicate 2; control for Ezh2-deficient MEFs
GSM580394 Wild Type MEFs; biological replicate 3; control for Ezh2-deficient MEFs
This SubSeries is part of SuperSeries:
GSE23659 Epigenetic antagonism between Snf5 and Ezh2 during oncogenic transformation and elevated levels of H3K27me3 in Snf5-deficient cells
Relations
BioProject PRJNA133129

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23656_RAW.tar 49.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap