|
Status |
Public on Jan 17, 2024 |
Title |
HeLa cells, Negative control, rep 2 |
Sample type |
SRA |
|
|
Source name |
HeLa cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa cells cell type: cervix epithelial passages: 5-8 treatment: Negative GapmeR (40 nM)
|
Treatment protocol |
Hela cells were treated with 80 uM cisplatin for 16 h
|
Growth protocol |
HeLa cells were routinely cultured in RPMI medium supplemented with 10% FBS and 1 % penicillin-streptomycin
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by TRIzol Reagent RNA samples were subjected to DNAse treatment and poly(A) enrichment Custom Illumina
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
160701_SND393_B_L002_GDU-32, 160701_SND393_B_L003_GDU-32
|
Data processing |
Adapter removal by Trim Galore! Mapping to the genome by STAR Read counting by featureCounts Differential expression analysis by DESeq2 Genome_build: GRCh38 Supplementary_files_format_and_content: raw_counts, normalized_counts_significant, results_significant_annotated
|
|
|
Submission date |
Jan 11, 2022 |
Last update date |
Jan 17, 2024 |
Contact name |
Bünyamin Akgül |
E-mail(s) |
bunyaminakgul@iyte.edu.tr
|
Organization name |
Izmir Institute of Technology
|
Department |
Molecular Biology and Genetics
|
Lab |
Non-coding RNA Laboratory
|
Street address |
Gulbahce Campus
|
City |
Izmir |
State/province |
Urla |
ZIP/Postal code |
35433 |
Country |
Turkey |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE193447 |
Death Receptor 5 Antisense Long Noncoding RNA and Cisplatin Modulate Macromolecular and Metabolic Changes in HeLa Cells |
|
Relations |
Reanalysis of |
GSM4869493 |
BioSample |
SAMN24836937 |
SRA |
SRX13719316 |