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| Status |
Public on Jan 17, 2024 |
| Title |
Death Receptor 5 Antisense Long Noncoding RNA and Cisplatin Modulate Macromolecular and Metabolic Changes in HeLa Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have performed a transcriptomics study in which we first apply cisplatin to HeLa cells. Total RNA was isolated from control as well as treated cells and apoptosis was confirmed by Annexin V and 7AAD staining in flow cytometry. Total RNAs were subjected to deep-sequencing to identify differentially expressed mRNAs. We then took advantage of bioinformatic tools to identify which pathways are affected by Cisplatin treatment
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| |
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| Overall design |
Total RNA was isolated from negative control and Cisplatin (80 uM) treated HeLa cells in triplicate to perform deep-sequencing. Negative controls from GSE160227 data was used as negative control in this study
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| Web link |
https://aperta.ulakbim.gov.tr/record/252114
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| |
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| Contributor(s) |
Gurer DC, Erdogan I, Ahmadov U, Sweef O, Akgul B |
| Citation(s) |
37529795 |
| |
| Submission date |
Jan 11, 2022 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Bünyamin Akgül |
| E-mail(s) |
bunyaminakgul@iyte.edu.tr
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| Organization name |
Izmir Institute of Technology
|
| Department |
Molecular Biology and Genetics
|
| Lab |
Non-coding RNA Laboratory
|
| Street address |
Gulbahce Campus
|
| City |
Izmir |
| State/province |
Urla |
| ZIP/Postal code |
35433 |
| Country |
Turkey |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA796277 |
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