|
| Status |
Public on Jan 17, 2024 |
| Title |
HeLa cells, Cisplatin, rep1 |
| Sample type |
SRA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa cells
cell type: cervix epithelial
passages: 5-8
treatment: Cisplatin (80 uM)
|
| Treatment protocol |
Hela cells were treated with 80 uM cisplatin for 16 h
|
| Growth protocol |
HeLa cells were routinely cultured in RPMI medium supplemented with 10% FBS and 1 % penicillin-streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by TRIzol Reagent
RNA samples were subjected to DNAse treatment and poly(A) enrichment
Custom Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
160701_SND393_B_L002_GDU-43, 160701_SND393_B_L003_GDU-43
|
| Data processing |
Adapter removal by Trim Galore!
Mapping to the genome by STAR
Read counting by featureCounts
Differential expression analysis by DESeq2
Genome_build: GRCh38
Supplementary_files_format_and_content: raw_counts, normalized_counts_significant, results_significant_annotated
|
| |
|
| Submission date |
Jan 11, 2022 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Bünyamin Akgül |
| E-mail(s) |
bunyaminakgul@iyte.edu.tr
|
| Organization name |
Izmir Institute of Technology
|
| Department |
Molecular Biology and Genetics
|
| Lab |
Non-coding RNA Laboratory
|
| Street address |
Gulbahce Campus
|
| City |
Izmir |
| State/province |
Urla |
| ZIP/Postal code |
35433 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE193447 |
Death Receptor 5 Antisense Long Noncoding RNA and Cisplatin Modulate Macromolecular and Metabolic Changes in HeLa Cells |
|
| Relations |
| BioSample |
SAMN24836935 |
| SRA |
SRX13719318 |
