|
| Status |
Public on Aug 21, 2024 |
| Title |
363 WT 3M H3K27ac |
| Sample type |
SRA |
| |
|
| Source name |
C57/BL6 mouse forebrain
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
cell type: NeuN+ neuronal nuclei
age: 3 months
chip antibody: H3K27ac
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Flash Frozen forebrains were extracted in nuclei extraction buffer, labelled with NeuN for 1hr then sorted with FACS. NeuN+ nuclei were pelleted, then split into ~200K aliquots for ChIP-seq. Nuclei were sonicated into 300bp fragments, then H3K4me3 or H3K27ac ChIP was performed.
Nugen Ovation Ultralow V2 library preparation - amplification for 17 cycles (H3K27ac), or 18 cycles (H3K4me3) 0347 V2 1-96/0344 V2 1-16.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
H3K27ac ChIP-seq from NeuN+ nuclei of 3m old C57/BL6 mice.
|
| Data processing |
Sequenced using HiSeq 2500 - mean read depth 13.9M H3K4me3, 16.2M H3K27ac.
Removed library tags.
Pseudoreplicates generated.
Mapped to MM10 using bowtie2.
bigwigs generated.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: bigwig files for pseudoreplicates.
|
| |
|
| Submission date |
Jan 19, 2022 |
| Last update date |
Aug 21, 2024 |
| Contact name |
Phillippa Claire Taberlay |
| E-mail(s) |
Phillippa.Taberlay@utas.edu.au, Phillippa.Taberlay@gmail.com
|
| Organization name |
University of Tasmania
|
| Department |
College of Health and Medicine
|
| Street address |
17 Liverpool St
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE190102 |
Ageing-Related Changes to H3K4me3, H3K27ac and H3K27me3 in Purified Mouse Neurons |
|
| Relations |
| BioSample |
SAMN25119292 |
| SRA |
SRX13830469 |
