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| Status |
Public on Aug 21, 2024 |
| Title |
Ageing-Related Changes to H3K4me3, H3K27ac and H3K27me3 in Purified Mouse Neurons |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Neurons are central to lifelong learning and memory, but ageing disrupts their morphology and function, leading to cognitive decline. Although epigenetic mechanisms are known to play crucial roles in learning and memory, neuron-specific genome-wide epigenetic maps into old age remain scarce, often being limited to whole-brain homogenates and confounded by glial cells. Here, we mapped H3K4me3, H3K27ac, and H3K27me3 in mouse neurons across their lifespan. This revealed stable H3K4me3 and global losses of H3K27ac and H3K27me3 into old age. We observed patterns of synaptic function gene deactivation, regulated through the loss of the active mark H3K27ac, but not H3K4me3. Alongside this, embryonic development loci lost repressive H3K27me3 in old age. This suggests a loss of a highly refined neuronal cellular identity linked to global chromatin reconfigu-ration. Collectively, these findings indicate a key role for epigenetic regulation in neurons that is inextricably linked with ageing.
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| Overall design |
H3K4me3 and H3K27ac ChIP-seq from 3, 6, 12, and 24m old Mus musculus forebrain neuronal nuclei sorted by FACS. Biological replicates (2-4 samples) for H3K4me3 and H3K27ac, and total input controls are included as fastq files, and analysed bigwigs.
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| Contributor(s) |
Giles KA, Phipps AJ, Cashion J, Huskins SN, Vickers JC, Mercer TR, Robinson MD, Woodhouse A, Taberlay PC |
| Citation(s) |
39195281 |
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| Submission date |
Dec 03, 2021 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Phillippa Claire Taberlay |
| E-mail(s) |
Phillippa.Taberlay@utas.edu.au, Phillippa.Taberlay@gmail.com
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| Organization name |
University of Tasmania
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| Department |
College of Health and Medicine
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| Street address |
17 Liverpool St
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| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7000 |
| Country |
Australia |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (43)
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| Relations |
| BioProject |
PRJNA798693 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE190102_H3K27ac_WT12_pseudo1.sorted.bw |
181.9 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT12_pseudo2.sorted.bw |
180.2 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT12_pseudo3.sorted.bw |
180.6 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT24_pseudo1.sorted.bw |
159.1 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT24_pseudo2.sorted.bw |
159.1 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT3_pseudo1.sorted.bw |
162.3 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT3_pseudo2.sorted.bw |
161.8 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT3_pseudo3.sorted.bw |
162.3 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT3_pseudo4.sorted.bw |
162.1 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT6_pseudo1.sorted.bw |
195.1 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT6_pseudo2.sorted.bw |
195.1 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K27ac_WT6_pseudo3.sorted.bw |
187.7 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT12_pseudo1.sorted.bam.bw |
117.5 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT12_pseudo2.sorted.bam.bw |
117.5 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT24_pseudo1.sorted.bw |
146.1 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT24_pseudo2.sorted.bw |
148.3 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT24_pseudo3.sorted.bw |
148.3 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT24_pseudo4.sorted.bw |
148.7 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT3_pseudo1.sorted.bw |
130.4 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT3_pseudo2.sorted.bw |
130.5 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT3_pseudo3.sorted.bw |
131.3 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT3_pseudo4.sorted.bw |
131.2 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT6_pseudo1.sorted.bw |
141.6 Mb |
(ftp)(http) |
BW |
| GSE190102_H3K4me3_WT6_pseudo2.sorted.bw |
141.6 Mb |
(ftp)(http) |
BW |
| GSE190102_RAW.tar |
3.6 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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