|
| Status |
Public on Jan 21, 2022 |
| Title |
CY |
| Sample type |
RNA |
| |
|
| Source name |
heart tissue, Cyclophosphamide
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: heart
|
| Treatment protocol |
Six-week-old female mice were intraperitoneally injected with cyclophosphamide (CY, 400 mg/kg), acrolein (5 mg/kg), or N-acetyl cysteine (200 mg/kg) + CY once a daily for two days. Control mice received only normal saline.
|
| Growth protocol |
C57BL/6J mice were housed individually in a temperature-controlled facility (22 ± 2 ℃) under a 12 hours light/dark cycle (light from 7:00 to 19:00). All mice were free access to water and food.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated from heart tissue using NucleoSpin® RNA/Protein kit (MACHEREY-NAGEL., Düren, Germany).
|
| Label |
Cy3
|
| Label protocol |
cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Mouse Gene Expression Microarray 8x60K v2 ; Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
|
| |
|
| Submission date |
Jan 20, 2022 |
| Last update date |
Jan 21, 2022 |
| Contact name |
Takuro Nishikawa |
| E-mail(s) |
adu44150@ams.odn.ne.jp
|
| Organization name |
Kagoshima University
|
| Department |
Pediatrics
|
| Street address |
Sakuragaoka 8-35-1
|
| City |
Kagoshima |
| ZIP/Postal code |
890-8520 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE194073 |
Effect of high-dose cyclophosphamide gene expression profiles in cardiomyocytes of C57BL/6J mice |
|
