|
| Status |
Public on Mar 20, 2023 |
| Title |
D8, iSOX18, No DOX, CD43plusCD235aplusCD41aplusCD45neg, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Day 8 differntiated iSOX18 H1 HSCs
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell type: Hematopoietic progenitors
cell surface markers: Day 8 CD43+CD34+CD235a+CD41a+CD45-
treatment: no DOX
genotype: no SOX18 induction
Stage: Day 8
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RNeasy micro kit (Qaigen), and used with 100 ng total RNA for preparing sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Data processing |
RNA-seq fragments were aligned by STAR (version 2.5.2b) to the human genome (hg38) with GENCODE basic gene annotations (version 38)
Gene expression levels were quantified by RSEM (version 1.3.0)
Genome_build: hg38
Supplementary_files_format_and_content: TSV: RSEM quantification results at the gene level
|
| |
|
| Submission date |
Jan 28, 2022 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Igor I. Slukvin |
| Organization name |
University of Wisconsin
|
| Street address |
1220 Capitol Court
|
| City |
Madison |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (1) |
| GSE195670 |
Quantitative analysis of tet inducible SOX18 during hematopoietic differentiation and Natural killer cell differentiation |
|
| Relations |
| BioSample |
SAMN25374644 |
| SRA |
SRX13971225 |
