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Sample GSM588112 Query DataSets for GSM588112
Status Public on Oct 01, 2010
Title Strain QX235 (Cy5) vs Common Reference (Cy3)
Sample type RNA
 
Channel 1
Source name GreenKRUSMS20071205_251506110243_S01.tif
Organism Caenorhabditis elegans
Characteristics strain name: Strain QX235
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label: Cy3
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name RedKRUSMS20071205_251506110243_S01.tif
Organism Caenorhabditis elegans
Characteristics strain source: Mixed strain, mixed stage, N2 and CB4856
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label: Cy5
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size: 5
Scan Date: 2007-12-05
Scan Time: 10:15:37
Scanner Make: Agilent Technologies Scanner
Scanner Model: G2505B
Scanning software: ChipScan
Scanning software version: A.7.0.1
Description Strain QX235 (Cy5) vs Common Reference (Cy3)
Data processing Extraction Software: Agilent Feature Extractor
Extraction Software Version: 9.5.3.1
Datafile type: Agilent result file
Agilent feature extraction software was used to perform dye bias correction and linear normalization.
 
Submission date Aug 27, 2010
Last update date Aug 31, 2010
Contact name Matthew Rockman
E-mail(s) mrockman@nyu.edu
Organization name New York University
Street address 100 Washington Sq. E.
City New York
State/province NY
ZIP/Postal code 10003
Country USA
 
Platform ID GPL7727
Series (1)
GSE23857 QX recombinant inbred advanced intercross lines of C. elegans

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 .516 95069.96 66484.95 27061.2 8410.567
2 0 11.61576 10.9236 25.8 20.33333
3 0 11.72389 11.09446 25.15385 21.10769
4 0 11.8225 11.25439 23.8 20.72308
5 0 11.91479 11.40846 23.96774 22.25806
6 0 11.99688 11.54978 22.3125 20.29688
7 0 12.07224 11.68393 23.91935 20.3871
8 0 12.13959 11.80844 25.14925 22.47761
9 0 12.19916 11.92326 25.25397 20.74603
10 0 12.25309 12.03222 23.61017 21.35593
11 0 12.2992 12.13054 22.67797 21.59322
12 -1.436 5254.903 14222.85 1420.371 1600.226
13 -.405 182.5462 241.7538 76.25806 49.87097
14 1.102 279.2008 130.1121 101.5902 37.47541
15 1.448 250.3052 91.75587 93.7931 33.2069
16 .176 227.7482 201.5953 87.75439 45.03509
17 -.283 12285.79 14947.47 3251.873 1634.444
18 -.765 299.0616 508.0873 106.3077 77.72308
19 -.7 2815.483 4573.857 765.7727 512.3939
20 -1.258 155.528 371.9175 68.64912 62.80702

Total number of rows: 44381

Table truncated, full table size 2014 Kbytes.




Supplementary file Size Download File type/resource
GSM588112.txt.gz 13.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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