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Sample GSM588171 Query DataSets for GSM588171
Status Public on Oct 01, 2010
Title Strain QX135 (Cy5) vs Common Reference (Cy3)
Sample type RNA
 
Channel 1
Source name GreensonjaWORMS_251506110081_S01.tif
Organism Caenorhabditis elegans
Characteristics strain name: Strain QX135
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label: Cy3
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name RedsonjaWORMS_251506110081_S01.tif
Organism Caenorhabditis elegans
Characteristics strain source: Mixed strain, mixed stage, N2 and CB4856
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label: Cy5
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size: 5
Scan Date: 2007-03-09
Scan Time: 12:12:57
Scanner Make: Agilent Technologies Scanner
Scanner Model: G2505B
Scanning software: ChipScan
Scanning software version: A.7.0.1
Description Strain QX135 (Cy5) vs Common Reference (Cy3)
Data processing Extraction Software: Agilent Feature Extractor
Extraction Software Version: 9.1.3.1
Datafile type: Agilent result file
Agilent feature extraction software was used to perform dye bias correction and linear normalization.
 
Submission date Aug 27, 2010
Last update date Aug 31, 2010
Contact name Matthew Rockman
E-mail(s) mrockman@nyu.edu
Organization name New York University
Street address 100 Washington Sq. E.
City New York
State/province NY
ZIP/Postal code 10003
Country USA
 
Platform ID GPL7727
Series (1)
GSE23857 QX recombinant inbred advanced intercross lines of C. elegans

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 .059 58067.13 55757.32 12855.13 6877.036
2 0 16.71349 18.05594 27.93548 21.90323
3 0 16.65499 18.03466 26.3871 21.62903
4 0 16.59898 18.01386 26.59016 23.62295
5 0 16.54617 17.99388 26.61905 21.33333
6 0 16.4963 17.97451 25.93651 22.04762
7 0 16.44858 17.95562 26.70769 22.64615
8 0 16.4044 17.93773 27.52941 21.48529
9 0 16.36224 17.92028 26.40984 21.21311
10 0 16.32221 17.90325 25.89552 21.34328
11 0 16.28506 17.88707 26.66667 23.71212
12 -1.889 5647.32 20922.27 1317.143 2625.778
13 -.029 333.6964 340.4356 105.5 66.70313
14 .298 400.6137 325.7975 121.1077 64.98462
15 .698 388.9859 239.7586 118.7586 54.36207
16 .885 418.8484 226.736 125.918 52.83607
17 .791 45086.22 26058.24 10407.87 3277.79
18 -.144 1040.763 1150.374 269.8621 168.431
19 -1.725 2173.552 7186.775 531.8833 923.3167
20 -1.186 217.5124 494.8456 80.47692 86.8

Total number of rows: 44381

Table truncated, full table size 2013 Kbytes.




Supplementary file Size Download File type/resource
GSM588171.txt.gz 11.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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