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Sample GSM588221 Query DataSets for GSM588221
Status Public on Oct 01, 2010
Title Strain QX34 (Cy3) vs Common Reference (Cy5)
Sample type RNA
 
Channel 1
Source name GreensonjaWORMS_251506110240_S01.tif
Organism Caenorhabditis elegans
Characteristics strain name: Strain QX34
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label: Cy5
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name RedsonjaWORMS_251506110240_S01.tif
Organism Caenorhabditis elegans
Characteristics strain source: Mixed strain, mixed stage, N2 and CB4856
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label: Cy3
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size: 5
Scan Date: 2007-11-30
Scan Time: 10:08:21
Scanner Make: Agilent Technologies Scanner
Scanner Model: G2505B
Scanning software: ChipScan
Scanning software version: A.7.0.1
Description Strain QX34 (Cy3) vs Common Reference (Cy5)
Data processing Extraction Software: Agilent Feature Extractor
Extraction Software Version: 9.1.3.1
Datafile type: Agilent result file
Agilent feature extraction software was used to perform dye bias correction and linear normalization.
 
Submission date Aug 27, 2010
Last update date Aug 31, 2010
Contact name Matthew Rockman
E-mail(s) mrockman@nyu.edu
Organization name New York University
Street address 100 Washington Sq. E.
City New York
State/province NY
ZIP/Postal code 10003
Country USA
 
Platform ID GPL7727
Series (1)
GSE23857 QX recombinant inbred advanced intercross lines of C. elegans

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (G_PROCESSED_SIGNAL/R_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 -0.36 37391.65 29137.24 15553.79 2331.429
2 0 8.900572 14.67343 23.15789 20.4386
3 0 8.967997 14.79122 24.61905 19.92063
4 0 9.030954 14.90259 25.87302 21.07937
5 0 9.087267 15.00349 23.60345 19.24138
6 0 9.137884 15.09585 24.43077 19.58462
7 0 9.183006 15.1798 23.1129 19.77419
8 1.7 9.222129 29.95507 24.27451 23.17647
9 0 9.257685 15.32371 25.24194 21.56452
10 0 9.287183 15.38337 23.55932 20.79661
11 0 9.313432 15.43818 26.01695 21.47458
12 -2.126 16388.15 3754.641 6463.404 300.5088
13 -0.553 310.0252 211.2728 149.8906 36.5
14 2.695 288.3943 1867.457 141.2969 159.2031
15 1.764 138.9496 472.0141 82.79365 55.68254
16 0.318 197.5604 246.3329 105.6984 38.93651
17 0.416 20016.24 26698.87 7845.563 1990.609
18 -0.03 914.1323 895.4717 385.629 86.72581
19 -1.671 6189.535 1944.291 2445.759 163.9138
20 -0.8 489.1943 281.049 219.9219 41.40625

Total number of rows: 44381

Table truncated, full table size 2041 Kbytes.




Supplementary file Size Download File type/resource
GSM588221.txt.gz 11.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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