NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM588271 Query DataSets for GSM588271
Status Public on Oct 01, 2010
Title Strain QX139 (Cy3) vs Common Reference (Cy5)
Sample type RNA
 
Channel 1
Source name GreensonjaWORMS_251506110141_S01.tif
Organism Caenorhabditis elegans
Characteristics strain name: Strain QX139
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label: Cy5
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name RedsonjaWORMS_251506110141_S01.tif
Organism Caenorhabditis elegans
Characteristics strain source: Mixed strain, mixed stage, N2 and CB4856
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label: Cy3
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size: 5
Scan Date: 2007-08-08
Scan Time: 10:59:40
Scanner Make: Agilent Technologies Scanner
Scanner Model: G2505B
Scanning software: ChipScan
Scanning software version: A.7.0.1
Description Strain QX139 (Cy3) vs Common Reference (Cy5)
Data processing Extraction Software: Agilent Feature Extractor
Extraction Software Version: 9.1.3.1
Datafile type: Agilent result file
Agilent feature extraction software was used to perform dye bias correction and linear normalization.
 
Submission date Aug 27, 2010
Last update date Aug 31, 2010
Contact name Matthew Rockman
E-mail(s) mrockman@nyu.edu
Organization name New York University
Street address 100 Washington Sq. E.
City New York
State/province NY
ZIP/Postal code 10003
Country USA
 
Platform ID GPL7727
Series (1)
GSE23857 QX recombinant inbred advanced intercross lines of C. elegans

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (G_PROCESSED_SIGNAL/R_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 -0.446 89686.69 65817.54 45426.59 12019.74
2 0 13.94326 13.04014 26.98462 21.26154
3 0 14.01057 13.1223 26.68182 21.86364
4 0 14.07388 13.20057 26.0339 20.77966
5 0 14.13112 13.27234 23.5625 22.40625
6 0 14.18282 13.33818 26.28125 23.46875
7 0 14.23073 13.40023 24.73333 19.93333
8 0 14.27241 13.45528 26.39683 21.4127
9 0 14.31068 13.50691 24.47273 20.50909
10 0 14.34423 13.55333 24.74603 21.04762
11 0 14.37364 13.59516 27.46667 21.73333
12 -1.147 6769.486 3057.682 3330.313 552.7656
13 -0.318 140.3427 112.559 98.65625 42.875
14 1.123 104.6096 227.7736 81.34848 62.72727
15 -1.685 79.44592 24.70793 69.22581 27.77419
16 0.657 85.87112 135.4017 72.43333 46.81667
17 0.4 10003.09 13195.33 4880.477 2285.708
18 -0.018 345.8178 341.5161 198.6102 82.11864
19 -1.733 3232.563 972.386 1596.833 189.9833
20 -0.666 84.01295 52.93993 72.04918 32.78689

Total number of rows: 44381

Table truncated, full table size 2026 Kbytes.




Supplementary file Size Download File type/resource
GSM588271.txt.gz 11.7 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap