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Sample GSM588274 Query DataSets for GSM588274
Status Public on Oct 01, 2010
Title Strain QX149 (Cy3) vs Common Reference (Cy5)
Sample type RNA
 
Channel 1
Source name GreenKRUSMS20071205_251506110249_S01.tif
Organism Caenorhabditis elegans
Characteristics strain name: Strain QX149
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label: Cy5
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name RedKRUSMS20071205_251506110249_S01.tif
Organism Caenorhabditis elegans
Characteristics strain source: Mixed strain, mixed stage, N2 and CB4856
Biomaterial provider Matt Rockman, Kruglyak Lab, Princeton University
Extracted molecule total RNA
Extraction protocol Extraction method: Trizol followed by Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label: Cy3
reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size: 5
Scan Date: 2007-12-06
Scan Time: 14:50:34
Scanner Make: Agilent Technologies Scanner
Scanner Model: G2505B
Scanning software: ChipScan
Scanning software version: A.7.0.1
Description Strain QX149 (Cy3) vs Common Reference (Cy5)
Data processing Extraction Software: Agilent Feature Extractor
Extraction Software Version: 9.5.3.1
Datafile type: Agilent result file
Agilent feature extraction software was used to perform dye bias correction and linear normalization.
 
Submission date Aug 27, 2010
Last update date Aug 31, 2010
Contact name Matthew Rockman
E-mail(s) mrockman@nyu.edu
Organization name New York University
Street address 100 Washington Sq. E.
City New York
State/province NY
ZIP/Postal code 10003
Country USA
 
Platform ID GPL7727
Series (1)
GSE23857 QX recombinant inbred advanced intercross lines of C. elegans

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (G_PROCESSED_SIGNAL/R_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 -0.344 81582.1 64254.14 18954.65 8120.183
2 0 13.50761 13.17552 24.22222 22.07937
3 0 13.68355 13.36879 22.06452 22.58065
4 0 13.84547 13.54744 22.73016 22.03175
5 0 13.99409 13.71224 22.59016 20.7541
6 0 14.13062 13.86439 24.44444 19.88889
7 0 14.25361 14.00208 21.10169 23.18644
8 0 14.36686 14.12953 21.13115 20.47541
9 0 14.46857 14.24469 23.88136 20.16949
10 0 14.55949 14.3484 22.28571 20.71429
11 0 14.64074 14.4418 23.31746 20.20635
12 -1.366 14078.06 5460.049 2986.415 638.8
13 -0.732 215.381 129.6509 74.53968 38.90476
14 1.957 255.1327 990.7293 82.5303 134.8182
15 1.297 109.5774 269.3207 52 54.25806
16 -0.027 204.6151 200.8454 71.50769 46.55385
17 0.044 16868.52 17392.49 3515.188 1943.609
18 -0.557 803.842 546.2402 194.7069 84.41379
19 -1.208 5472.971 2369.524 1155.905 284.5556
20 -1.344 484.6477 190.9101 128.3906 45.15625

Total number of rows: 44381

Table truncated, full table size 2039 Kbytes.




Supplementary file Size Download File type/resource
GSM588274.txt.gz 13.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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